Knaresborough Town will represent the Toolstation North Eastern Counties League at today's inaugural Toolstation Cup (3pm).
Knaresborough will be taking on Bristol Manor Farm, who came second in the Toolstation Western League in the 2013/14 season.
Knaresborough Town have been selected to represent the League after beating Eccleshill United to win the NECL League Cup.
Manager Brian Davey said: "It is a great honour for our club to represent our league against Bristol Manor.
It will be a good test for us during pre-season."
The game has been set up to raise money for the RNLI Flood Rescue Team and Asthma UK.
Entry for the match at Staveley Miners Welfare is free, but donations will be collected.