To allow organisers to begin setting up the extensive event village, University Road, in York, will be closed between Innovation Way and Green Dykes Lane on Saturday from noon and will re-open at 9pm after the race.
Elsewhere, closures on the Sunday begin from 6am but will re-open on a rolling basis as soon as it is safe to do so.
City centre roads affected by the closures include Walmgate, Hope Street, Leadmill Lane, Piccadilly, Parliament Street, Blake Street, Duncombe Place, Deangate, Goodramgate and Monkgate.
Most will close from around 9.15am and should be re-opened by around 10.30am.
Further afield, Heworth Green A1036, Stockton Lane, The Village, Common Lane, Northgate Lane, Holtby Lane, Murton Way, Bad Bargain Lane and Osbaldwick Lane will be among those affected.
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